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This work was funded in part by NSF grant
#0213764 and
by the Initiative for Future Agriculture and Food Systems Grant no. 2001-52100-11292 from the USDA Cooperative State Research, Education, and Extension Service.
Latest release: FPC V9.1 (3 Jul 2008);
Publication of paper on HICF in Plant Physiology, see references
FPC downloads, tutorials and documentation
FPC takes as input a set of clones and their restriction fragments
(called Bands) and assembles the clones into contigs; for more information,
see summary.
FPC contributions are
by the GSC, BCGSC, Sanger Centre, Clemson University, and University
of Arizona.
FPC V9.1 release (July 2008, details) 
| Download
| Documentation
| Description
|
| FPC
| Documentation Guide
| FPC tar file, executables, and user's guide
|
| demo.tar
| FPC Tutorial* (.doc)
| Tutorial of salient features
|
| ctgdemo.tar
| Contig display tutorial
| Tutorial on new contig display features
|
| bssdemoV2.tar.gz
| BSS tutorial
| Tutorial on BSS (runs BLAST/BLAT and adds results to FPC)
|
| NEW seqdemo.tar.gz
| Sequence track tutorial
| Tutorial on using the FPC draft sequence alignment functions
|
| mtpdemoV2.tar
| MTP tutorial
| Tutorial on automatically select minimal tiling path in FPC
|
| hicf_auto.tar
| HICF & Auto Merge tutorial
| Tutorial on assembling HICF and
performing auto merging
|
| fsd.tar
| FSD and
FSD2 Manual
| Program to extract Sequence and FPC Simulated Digest
|
*Published in Ian Dunham (ed) Genomic Mapping
and Sequencing, Horizon Press, Genome Technology series.
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Web-based FPC Maps
A Web based Java program displays the contigs for the following FPC databases:
Also see:
- GSC: human,
mouse, Arabidopsis thaliana, Salmonella enterica.
- BCGSC: human, mouse, rat,
bovine, C.neoformans.
- Sanger:
mouse-human synteny.
- TAMU: soybean, arabidopsis,
chicken, indica rice.
- UCDavis:
wheat, potato.
- Gramene: map viewer for comparative
mapping (Maps Viewer).
- NCBI: mouse
map (see Maps&Options).
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References
Nelson, W., J. Dvorak, M. Luo, J. Messing, R. Wing, and C. Soderlund (2006). Efficacy of clone fingerprinting methodologies. Genomics 89:160-165.
Download
Nelson, W, A Bharti, E Butler, F Wei, G Fuks, H Kim,
R Wing, J Messing, and C Soderlund (2005).
Whole-Genome Validation of High-Information-Content Fingerprinting. Plant Physiology
139:27-38.
Download
Nelson, W. and C. Soderlund (2005). Software for restriction fragment physical
maps. K. Meksem, G. Kahl (ed) The Handbook of Genome Mapping: Genetic
and Physical Mapping, Wiley-VCH. pp. 285-306.
Download
Engler,
F., J. Hatfield, W. Nelson, and C. Soderlund (2003). Locating sequence
on FPC maps and selecting a minimal tiling path. Genome Research
13:2152:2163. Download
Supplemental
Engler,
F. and C. Soderlund (2002). Software for Physical Maps. In Ian Dunham
(ed) Genomic Mapping and Sequencing, Horizon Press, Genome
Technology series. Norfolk, UK, pp. 201-236.
Soderlund,
C., F. Engler, J. Hatfield, S. Blundy, M. Chen, Y. Yu, and R. Wing (2002).
Mapping sequence to Rice FPC. In P. Wang, J. Wang and C. Wu (ed)
Computational Biology and Genome Informatics. World
Scientific Publishing. pp 59-79.
Download.
Ness,
S.R., Terpstra, W., Krzywinski, M., Marra M., Jones, A., and Ness,
S.J.M. (2002). Assembly of fingerprint contigs: parallelized FPC.
BioInformatics 18:484-485.
Chen,
M., Presting, G., Barbazuk, W., Goicoechea, J., Blackmon, B., Fang,
G., Kim, H., Frisch, D., Yu, Y., Higingbottom, S., Phimphilai, J.,
Phimphilai, D., Thurmond, S., Gaudette, B., Li, P., Liu, J., Hatfield,
J., Sun, S., Farrar, K., Henderson, C., Barnett, L., Costa, R.,
Williams, B., Walser, S., Atkins, M., Hall, C., Bancroft, I., Salse,
J., Regad, F., Mohapatra, T., Singh, N., Tyagi, A., Soderlund, C.,
Dean, R., and Wing, R. (2002). An integrated physical and genetic
map of the rice genome. Plant Cell.
The
International Human Genome Mapping Consortium (2001) A physical
map of the human genome, Nature 409:934-941
Download.
Soderlund,
C., S. Humphrey, A. Dunhum, and L. French (2000). Contigs built
with fingerprints, markers and FPC V4.7. Genome Research
10:1772-1787.
Download.
Soderlund,
C., S. Gregory and I. Dunham (1998). Sequence ready clones. In Bishop,
M.J. (ed) Guide to Human Genome Computing, Academic Press.
Mungal,
A., S. Humphray, S. Ranby, C. Edwards, R. Heathcott, C. Clee, E.
Holloway, A. Peck, P. Harrision, L. Green, A. Butler, C. Langford,
R. Gwilliam, E. Huckle, L. Baron, A. Smith, M. Leversha, Y. Ramsey,
S. Clegg, C. Rice, G. Maslen, S. Hunt, C. Scott, S. Soderlund, A.
Theaker, N. Carter, M. Ross, P. Deloukas, D. Bentley, I. Dunham
(1997) From Long Range Mapping to Sequence Ready Contigs on Human
Chromosome 6. DNA sequence - The Journal of Sequencing and Mapping,
8:151-154.
Soderlund,
C., I. Longden and R. Mott (1997) FPC: a system for building contigs
from restriction fingerprinted clones. CABIOS, 13: 523-535.
Download.
Gregory,
S., C. Soderlund and A. Coulson (1996) Contig assembly by fingerprinting.
In P. H. Dear (ed) Genome Mapping: a Practical Approach.
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