Elm leaf beetle

This work has been funded in part by the Deutsche Forschungsgemeinschaft (Me 1810/4-1.2 and Fe 778/1-1.2)
and the Max Planck Society.

Reference:
Kerstin Buechel ^1,2, Eric McDowell³, Will Nelson⁴, Anne Descour⁴, Monika Hilker¹, Jonathan Gershenzon², Trevor Fenning ⁵, Carol Soderlund⁴, David R. Gang³ and Torsten Meiners¹. (2012) An elm EST databases for detection of leaf beetle oviposition-induced defense genes. BMC Genomics 13:242. Link

¹ The Meiners lab, Freie Universitat Berlin, Germany

² The Max Planck Institute, Germany

³ The Gang lab, Washington State University, USA

⁴ The Soderlund lab, University of Arizona, USA

This study produced five cDNA libraries in order to study the oviposition-induced changes in the transcription profile of the elm tree. A total of 361,196 ESTs were sequenced using 454 technology and assembled using the PAVE software.

cDNA Libraries ESTs Contigs Unique contigs Singletons

Untreated control (C) 2,132 836 31 174
Egg & feeding (EF) 1,921 826 50 211
Feeding (F) 4,725 1,453 65 326
Methyl jasmonate (MeJA) 7,080 2,272 153 679
Transferred eggs (E) 2,133 811 40 188
EF + F 169,672 15,083 2,844 11,560
C + JA + E 71,239 9,141 860 8,043
C + EF+ F + JA + E 98,210 12,402 2,249 9,755
Tag unidentifiable 4,084 597 200 397
Total 361,196 21,490 - 31,333
SR4 clone 38,803 8,289 1,649 5,966
Total 399,999 23,139 - 37,299

Email Comments To: pave@agcol.arizona.edu