The University of Arizona
Elm leaf beetle
transcriptome database
 

This work has been funded in part by the Deutsche Forschungsgemeinschaft (Me 1810/4-1.2 and Fe 778/1-1.2)
and the Max Planck Society.

Reference:
Kerstin Buechel 1,2, Eric McDowell3, Will Nelson4, Anne Descour4, Monika Hilker1, Jonathan Gershenzon2, Trevor Fenning 5, Carol Soderlund4, David R. Gang3 and Torsten Meiners1. (2012) An elm EST databases for detection of leaf beetle oviposition-induced defense genes. BMC Genomics 13:242. Link

1 The Meiners lab, Freie Universitat Berlin, Germany
2 The Max Planck Institute, Germany
3 The Gang lab, Washington State University, USA
4 The Soderlund lab, University of Arizona, USA

This study produced five cDNA libraries in order to study the oviposition-induced changes in the transcription profile of the elm tree. A total of 361,196 ESTs were sequenced using 454 technology and assembled using the PAVE software.

cDNA Libraries ESTs Contigs Unique contigs Singletons
Untreated control (C)2,13283631174
Egg & feeding (EF)1,92182650211
Feeding (F) 4,7251,45365326
Methyl jasmonate (MeJA)7,0802,272153679
Transferred eggs (E) 2,133 811 40 188
EF + F 169,672 15,083 2,844 11,560
C + JA + E 71,239 9,141 860 8,043
C + EF+ F + JA + E 98,210 12,402 2,249 9,755
Tag unidentifiable 4,084 597 200 397
Total 361,196 21,490 - 31,333
SR4 clone 38,803 8,289 1,649 5,966
Total 399,999 23,139 - 37,299

 
Email Comments To: pave@agcol.arizona.edu